3-Methylcytosine (3-mC) antibody (pAb)

RRID: AB_2793510
Catalog No: 61111 Format: 100 µg $445 Buy
Catalog No: 61112 Format: 10 µg $110 Buy

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Antibody Type:
Protein A Chromatography
Human, Mouse, Not Species Specific


Immunofluorescence Validated Dot Blot Validated Immunocytochemistry Validated

Application Notes

Validated Applications:
DB: 0.1 - 1 µg/ml dilution

Published Applications:
Dot Blot
Please see references for more information. Individual optimization may be required.


This 3-methylcytosine antibody was raised against 3-methylcytidine conjugated to KLH and recognizes 3-methylcytosine.


Purified rabbit IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.



3-Methylcytosine (3-mC, 3-methylcytidine) antibody tested by dot blot analysis.
BSA conjugated nucleosides (starting at 10ng as indicated) were spotted onto PVDF membrane and blotted with 3-methylcytidine antibody at a dilution of 1:10,000.
Lane 1: 3-methylcytidine.
Lane 2: Cytidine.
Lane 3: 5-methylcytidine.
Lane 4: 5-hydroxymethylcytidine.


Methylation of DNA can occur non-enzymatically at the nitrogen-three of the cytosine base through spontaneous exposure to endogenous S-adenosyl methionine (SAM). The resulting 3-methylcytosine (3-mC) is mutagenic and must be repaired, which occurs in humans through the base excision repair (BER) or dealkylation via human homologues of the E. coli AlkB protein. 3-methylcytosine is present in human cell lines and increased levels of 3-mC impair proliferation.


Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.


This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting