Recombinant ALKBH2 protein

Catalog No: 81129 Format: 100 µg $495 Buy
Catalog No: 81829 Format: 1 mg $3,000 Buy

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Expressed In: E. coli Protein Species: Human


A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.


ALKBH2 (AlkB Homolog 2, Alpha-Ketoglutarate Dependent Dioxygenase) is a member of ALKBH protein family. It can repair alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. ALKBH2 can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. It can release modified bases from both DNA and RNA. The oxidative demethylation requires molecular oxygen, alpha-ketoglutarate and iron.

Application Notes

Recombinant ALKBH2 protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.

Protein Details

Full length ALKBH2 protein (accession number NP_001001655.1) was expressed in E. coli cells with an N-terminal 6xHis Tag. The molecular weight of ALKBH2 is 33.1 kDa.


Recombinant ALKBH2 protein
10% SDS-PAGE Coomassie staining
MW: 33.1 kDa
Purity: >82%

ALKBH2 dioxygenase activity assay
1 µg of ssDNA oligos (5’-AAAGCAG(1mA)ATTCGAAAAAGCGAAA-3’) was incubated with 0 µg, 0.5 µg, 1 µg, 2 µg, 4 µg of ALKBH2 in 50 µl reaction system including 50 mM HEPES-NaOH pH 8.0, 50 μM Fe(NH4)2(SO4)2, 1 mM 2-oxoglutarate, 2 mM ascorbate and 1 mM TCEP for 30 min at 37°C. Then ssDNA oligos were annealed with equimolar of non-methylated complement strand followed by 1 µg EcoRI digestion for 45 min at 37°C. 1/4 reaction products were run on a 20% Native PAGE gel and stained by ethidium bromide.


Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.


This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.