Recombinant ALKBH3 protein

Catalog No: 81130 Format: 100 µg $495 Buy
Catalog No: 81830 Format: 1 mg $3,000 Buy

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Expressed In: E. coli Protein Species: Human


A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.


ALKBH3 (AlkB Homolog 3, Alpha-Ketoglutarate Dependent Dioxygenase) is a member of ALKBH protein family. It mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). It can repair alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation. ALKBH3 has a strong preference for single-stranded DNA. It is able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promoting DNA unwinding to generate single-stranded substrate needed for ALKBH3. ALKBH3 also acts on RNA via demethylating N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5-untranslated regions (UTRs) and in the vicinity of start codons. The oxidative demethylation requires molecular oxygen, alpha-ketoglutarate and iron.

Application Notes

Recombinant ALKBH3 protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.

Protein Details

Full length ALKBH3 protein (accession number NP_631917.1) was expressed in E.coli cells with an N-terminal 6xHis Tag. The molecular weight of ALKBH3 is 36.9 kDa.


Recombinant ALKBH3 protein
12.5% SDS-PAGE Coomassie staining
MW: 36.9 kDa
Purity: >90%

ALKBH3 dioxygenase activity
1 µg of ssDNA oligos (5’-AAAGCAG(1mA) ATTCGAAAAAGCGAAA-3’) was incubated with varying concentrations of ALKBH3 in a reaction system including 50 mM HEPES-NaOH pH 8.0, 50 µM Fe(NH4)2(SO4)2, 1 mM 2-oxoglutarate, 2 mM ascorbate and 1 mM TCEP for 30 min at 37°C. Then ssDNA oligos were annealed with equimolar of non-methylated complement strand followed by 1 µg EcoRI digestion for 45 min at 37°C.

% reaction products were run on a 20% Native PAGE gel and stained by ethidium bromide.


Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.


This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.