KLF6 antibody (pAb)

Aliases: GBF, ZF9, BCD1, CBA1, CPBP, PAC1, ST12, COPEB
Catalog No: 61243 Format: 100 µg $435 Buy
Catalog No: 61244 Format: 10 µg $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Protein A Chromatography
Host:
Rabbit
Molecular Weight:
40 kDa
Reactivity:
Mouse

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 0.5 - 2 µg/ml dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Immunogen

This antibody was raised against a peptide within the internal region of human KLF6.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

References

 
KLF6 antibody (pAb) tested by Western blot.

KLF6 pAb tested by Western blot.
3T3-L1 nuclear extract extract following stimulation with a defined hormonal mixture (DMSO, dexamethasone, insulin, and IBMX) at 30 µg per lane was probed with KLF6 pAb at a dilution of 2 µg/ml .
    Lane 1: Day 0.
    Lane 2: Day 10 - fully differentiated adipocytes.

Background

KLF6 (Kruppel-like factor 6) is member of the Kruppel-like family of transcription factors and is a zinc finger protein that acts as a transcriptional activator and functions as a tumor suppressor. Could play a role in B-cell growth and development. Multiple transcript variants encoding different isoforms have been found for this gene, some of which are implicated in carcinogenesis.

Positive Control

Active Motif's 3T3-L1 nuclear extract (Catalog No. 36071) can be used as a positive control for KLF6 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting