KLF5 antibody (pAb)

RRID: AB_2614941
Aliases: CKLF, IKLF, BTEB2
Catalog No: 61099 Format: 100 µl $455 Buy
Catalog No: 61100 Format: 10 µl $110 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
65 kDa
Reactivity:
Human, Mouse

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes

Applications Validated by Active Motif:
ChIP: 5 - 10 µl per ChIP
ChIP-Seq: 5 - 10 µl each
ICC/IF: 1:2,000 dilution
WB*: 1:500 - 1:5,000 dilution

ChIP-Seq validation was performed by Active Motif's Epigenetics Services; the complete data set is available in the UCSC Genome Browser by clicking here.

*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP-qPCR
FC

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This KLF5 antibody was raised against a peptide derived from the N-terminus of mouse KLF5 (Krueppel-like factor 5).

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

KLF5 antibody (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from an adenocarcinoma cell line and 5 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 24 million sequence tags were mapped to identify KLF5 binding sites. The image shows binding across a region of chromosome 2. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

KLF5 antibody (pAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with KLF5 antibody at a 1:2,000 dilution.

KLF5 antibody (pAb) tested by Western Blot.
Nuclear extract of K-562 cells (20 µg) probed with the KLF5 antibody (pAb) at a dilution of 1:1,000.

Background

KLF5 (Kruppel-like factor 5, IKLF, Basic transcription element-binding protein 2, BTEB2) is a zinc-finger transcription factor that plays a role in cell proliferation, differentiation, cell cycle regulation, angiogenesis, and carcinogenesis and is especially important in epithelial cells. KLF5 (along with KLF2 and KLF4) are highly expressed in undifferentiated mouse embryonic stem cells and downregulated during early stages of differentiation. KLF5 and KLF2 can substitute for KLF4 in the reprogramming of differentiated cells into stem cells. KLF5 has a pro-proliferative role in cells and loss of both alleles of KLF5 is embryonic lethal.

Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for KLF5 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with KLF5 antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting