KLF4 antibody (pAb)

RRID: AB_2614947
Aliases: EZF, GKLF
Catalog No: 39745 Format: 100 µl $445 Buy
Catalog No: 39746 Format: 10 µl $110 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
55 kDa
Reactivity:
Human, Wide Range Predicted

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:2,000 - 1:5,000 dilution

Published Applications: see references

Immunogen

This KLF4 antibody was raised against recombinant full-length human KLF4.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 

KLF4 antibody tested by Western blot.
Lane 1: Nuclear extract (20 µg) of Hct116 cells.
Lane 2: Nuclear extract (20 µg) of primary human keratinocyte cells. Both probed with KLF4 antibody at a 1:5,000 dilution.

Background

KLF4 (Kruppel-like factor 4) is a transcription factor that binds GC-rich DNA sequences having a consensus core DNA binding sequence of CACCC. It is a member of the Kruppel-like factor (KLF) family, highly expressed in the gastrointestinal tract, skin and other epithelial tissues. Krüppel-like factors are a family of zinc-finger transcription factors that play important roles in many fundamental biologic processes including development, proliferation, differentiation, and apoptosis. KLF4 can function both as an activator and repressor of transcription, depending on the gene targeted. Generally, KLF4 serves as a cell cycle regulator, and it may be a tumor suppressor. KLF4 also appears to function as an oncogene in some situations. Additionally, overexpression of KLF4 (along with Oct3/4, Sox2 and c-Myc) can transform mouse fibroblasts into a state resembling embryonic stem cells (ES cells), referred to as Induced Pluripotency.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting