Histone H3K9ac antibody (mAb)
|Catalog No: 61251||Format: 100 µg||$455||Buy|
|Catalog No: 61952||Format: 50 µg||$265||Buy|
|Catalog No: 61252||Format: 10 µg||$110||Buy|
ChIP: 2 - 5 µg per ChIP
ChIP-Seq: 5 µg each
WB: 0.2 - 2 µg/ml dilution
NGS-QC® certification. This antibody has been processed by the NGS-QC® generator. For additional details, click here.
For Histone H3K9ac, we also offer AbFlex® Histone H3K9ac Recombinant Antibody (rAb). For details, see Catalog No. 91103.
The Histone H3 acetyl Lys9 antibody was raised against a peptide including acetyl-lysine 9 of human Histone H3.
Purified IgG in 0.1M Tris, pH 8.0, 1 M NaCl with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Acetylation of Lys9 is associated with trascriptional activation of genes.
Active Motif's Human Positive Control Primer Set ACTB-2 (Cat. No. 71005) and Human Negative Control Primer Set 1 (Cat. No. 71001) have been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Positive Control Primer Set Actb-2 (Cat. No. 71017) and Mouse Negative Control Primer Set 1 (Cat. No. 71011) have been validated for both qPCR and endpoint PCR.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K9ac antibody (mAb).
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting