5-Methylcytosine (5-mC) antibody (pAb)

Catalog No: 61255 Format: 100 µl $435 Buy
Catalog No: 61256 Format: 10 µl $105 Buy

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Antibody Type:
Protein A Chromatography
Molecular Weight:
Not Applicable
Other (Not Species Specific)


ChIP Validated Methyl-DNA Immunoprecipitation Validated Dot Blot Validated Immunohistochemistry Validated

Application Notes

Validated Applications:
MeDIP: 2 - 10 µg per IP
DB: 2 µg/ml dilution

Published Applications:

See references for more information. Individual optimization may be required.


This 5-Methylcytosine (5-mC) antibody was raised against 5-Methyl-cytidine conjugated to KLH and recognizes 5-Methylcytosine.


Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.


5-Methylcytosine (5-mC) antibody (pAb) tested by MeDIP analysis.

5-Methylcytosine antibody (pAb) tested by Methyl-DNA Immunoprecipitation (MeDIP).
Human DNA (500 ng) digested with MseI and denatured was subjected to MeDIP and then analyzed by quantitative real-time PCR with primers specific for genes that are methylated (KIAA0853, GEMIN4) or unmethylated (Untr12). The amount of MeDIP'd DNA (enriched DNA) was plotted.

5-Methylcytosine (5-mC) antibody (pAb) tested by dot blot analysis.

5-methylcytidine antibody (pAb) tested by dot blot analysis.
DNA from the Methylated DNA Standard Kit (Catalog No. 55008) were spotted on to a positively charged nylon membrane and blotted with 5-methylcytidine antibody recognizing 5-methylcytosine (2 µg/ml dilution).

Lane 1: single stranded unmethylated DNA.
Lane 2: single-stranded DNA containing 5-methylcytosine.
Lane 3: single-stranded DNA 5-hydroxymethylcytosine.
Lane 4: double-stranded unmethylated DNA.
Lane 5: double-stranded DNA containing 5-methylcytosine.
Lane 6: double-stranded DNA containing 5-hydroxymethylcytosine.


5-Methylcytosine (5-Methylcytidine) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. The 5-methylcytosine antibody has been developed to discriminate between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis.

qPCR Primers

Active Motif's Human Positive Control Primer Set ZC3H13 (Cat. No. 71009) and Human Negative Control Primer Set 1 (Cat. No. 71001) have been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Negative Control Primer Set 1 (Cat. No. 71011) has been validated for both qPCR and endpoint PCR.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with 5-Methylcytosine (5-mC) antibody (pAb).


Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.


This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting