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AbFlex® 5-methylcytosine antibody (rAb)

Aliases: 5-mC, 5-Methylcytosine
Catalog No: 91187 Format: 100 µg $375 Buy Now
Catalog No: 91188 Format: 10 µg $75 Buy Now

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Antibody Type:Recombinant
Reactivity:Other (Not Species Specific)


Dot Blot Validated

for AbFlex 5-methylcytosine antibody (rAb)Application Notes

Validated Applications:
Dot Blot: 1 µg / mL

Application Testing: Parental Monoclonal vs. Recombinant
View PDF file

AbFlex™ recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones.  For details on the parental clone, see Catalog No. 61479.

for AbFlex 5-methylcytosine antibody (rAb)Immunogen

This antibody was raised against 5-methylcytosine conjugated to KLH.

for AbFlex 5-methylcytosine antibody (rAb)Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 35 mM NaOAc, 0.035% sodium azide, and 30% glycerol.

for AbFlex 5-methylcytosine antibody (rAb)References

AbFlex<sup>®</sup> 5-methylcytosine antibody (rAb) tested by dot blot analysis.

Dot blot of AbFlex® 5-methylcytosine antibody (rAb). DNA standards from Cat# 55008 (5, 1 and 0.2 ng as indicated) were spotted onto positively charged nylon membrane and blotted with AbFlex 5-methylcytosine recombinant antibody at a 1 µg/mL.  
Lane 1: Unmethylated DNA.
Lane 2: DNA containing 5-methylcytosine. 
Lane 3: DNA containing 5-hydroxymethylcytosine.

for AbFlex 5-methylcytosine antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-Tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.

AbFlex® 5-methylcytosine (5-mC) antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. 5-Methylcytosine (5-Methylcytidine) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. This antibody is useful for discrimination between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis.

for AbFlex 5-methylcytosine antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex 5-methylcytosine antibody (rAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

AbFlex® 5-methylcytosine antibody (rAb)

Data Thumbnails

AbFlex<sup>®</sup> 5-methylcytosine antibody (rAb) tested by dot blot analysis.

Dot blot of AbFlex® 5-methylcytosine antibody (rAb).
(Click image to enlarge and see details.)