AbFlex® 5-methylcytosine antibody (rAb)

Aliases: 5-mC, 5-Methylcytosine
Catalog No: 91187 Format: 100 µg $385 Buy
Catalog No: 91188 Format: 10 µg $80 Buy

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Antibody Type:
Recombinant
Isotype:
IgG2a
Purification:
Protein A Chromatography
Host:
Mouse
Reactivity:
Other (Not Species Specific)

Applications

Dot Blot Validated

Application Notes


Validated Applications:
Dot Blot: 1 µg / mL

Immunogen

This antibody was raised against 5-methylcytosine conjugated to KLH.

Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 35 mM NaOAc, 0.035% sodium azide, and 30% glycerol.

References

 
AbFlex<sup>®</sup> 5-methylcytosine antibody (rAb) tested by dot blot analysis.

Dot blot of AbFlex® 5-methylcytosine antibody (rAb). DNA standards from Cat# 55008 (5, 1 and 0.2 ng as indicated) were spotted onto positively charged nylon membrane and blotted with AbFlex 5-methylcytosine recombinant antibody at a 1 µg/mL.  
Lane 1: Unmethylated DNA.
Lane 2: DNA containing 5-methylcytosine. 
Lane 3: DNA containing 5-hydroxymethylcytosine.

Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-Tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.

AbFlex® 5-methylcytosine (5-mC) antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. 5-Methylcytosine (5-Methylcytidine) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. This antibody is useful for discrimination between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting