TDG / Thymine-DNA glycosylase antibody (pAb)

Aliases: Jza1, JZA-3, E130317C12Rik
Catalog No: 61437 Format: 100 µl $435 Buy
Catalog No: 61438 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Protein A Chromatography
Host:
Rabbit
Molecular Weight:
55 kDa
Reactivity:
Mouse

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:1,500 dilution

Immunogen

This antibody was raised against a full-length recombinant protein corresponding to mouse thymine DNA glycosylase (TDG).

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
TDG / Thymine-DNA glycosylase antibody (pAb) tested by Western blot.

TDG / Thymine-DNA glycosylase antibody (pAb) tested by Western blot
Nuclear extract of P19 cells (30 µg) probed with TDG / Thymine-DNA glycosylase antibody (1:500).

Background

In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. In addition, TET proteins oxidize 5- methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5- formylcytosine (5fC), and 5-carboxylcytosine (5caC). TDG / Thymine-DNA glycosylase functions to correct G/T mispairs to G/C pairs and plays a role in active DNA demethylation by recognizing 5fC and 5caC and converting these to cystosine through the base excision repair (BER) pathway.

Positive Control

Active Motif's P19 nuclear extract (Catalog No. 36074) can be used as a positive control for TDG / Thymine-DNA glycosylase antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting