Secondary Antibody Conjugates
anti-rabbit & anti-mouse secondaries for enhanced HRP based detection
Active Motif's secondary antibody conjugates offer an improved method for the detection of primary antibodies. Having developed many quality primary antibodies, we found that the quality of secondary antibodies was also key to producing superior results. We therefore offer these secondary antibodies, conjugated to HRP for detecting primary antibodies bound to their targets. To conjugate the dyes or other labels to primary antibodies and other proteins, we offer our Sortag-IT™ Labeling Kits.
Figure 1: Multi-color staining of Phospho H2AX and tubulin using Chromeo 488 and Chromeo 642 secondary antibodies in untreated and etoposide-treated HeLa cells.
Active Motif's ATTO dye conjugates have been maximally cross-adsorbed against IgG's of a variety of species to eliminate background caused by non-specific binding. The fluorescent ATTO secondary antibody conjugates and the fluorescent Chromeo™ 488, Chromeo™ 505 and Chromeo™ 494 secondary antibody conjugates have been certified by Leica Microsystems for STimulated Emission Depletion (STED) microscopy, as they exhibited superior staining when compared to other fluorescent antibody conjugates.