alpha Tubulin antibody (mAb)

RRID: AB_2793243
Clone: 5-B-1-2
Catalog No: 39527 Format: 100 µl $435 Buy
Catalog No: 39528 Format: 10 µl $105 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Ascites
Host:
Mouse
Molecular Weight:
52 kDa
Reactivity:
Human, Mouse, Wide Range Predicted

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:2,500 dilution
ICC/IF: 1:1,000 - 1:10,000 dilution



Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Immunogen

This alpha Tubulin antibody was raised against total protein purified from sea urchin sperm axonemes.

Buffer

Ascites containing 30% glycerol and 0.035% sodium azide.

References

 

alpha Tubulin mAb (Clone 5-B-1-2) tested by immunofluorescence.
Top: HeLa cells stained with alpha Tubulin mAb at 1:500 dilution (red). Middle: Same cells stained with DAPI (blue). Bottom: Both images merged. Staining was carried out using MAX Stain™ Immunofluorescence Tools.

alpha Tubulin mAb (Clone 5-B-1-2) tested by Western blot.
HeLa whole-cell extract (15 µg/lane) probed with alpha Tubulin mAb (1:5,000 dilution).

Background

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of α-tubulin and β-tubulin. Alpha Tubulin expression is reduced in cells deficient in MeCP2, leading to conditions such as Rett syndrome and autistic-spectrum disorders.

Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for alpha Tubulin antibody (Clone 5-B-1-2).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with alpha Tubulin antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting