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Tissue Prep for NGS Assays

Tissue sample preparation for CUT&RUN, CUT&Tag, or CUT&Tag R-loop Assays

 

Tissue Prep for NGS Assays Kit Overview

Tissue Prep for NGS Assays Ki
 

Sample preparation is a critical step for any assay, and making sure nuclei are intact for CUT&RUN or CUT&Tag assays is key for successful results. The Tissue Prep for NGS Assays kit is optimized for processing tissue to yield nuclei for ChIC/CUT&RUN and CUT&Tag assays.

Tissue Prep for NGS Assays includes Lysis Buffer, Protease Inhibitor Cocktail, Strainers, and 1 X Wash Buffer optimized to yield intact nuclei for the ChIC/CUT&RUN and CUT&Tag-IT® R-loop Assays for the preparation of 24 samples. These components are also in the original CUT&Tag-IT® Assay Kit – Tissue (16 rxn).

How Tissue Prep for NGS Assays Works

The protocol guides through steps to homogenize tissue samples. Simply:

Tissue Prep for NGS Assays Highlights

  • Reagents included for 24 samples of 10 mg per assay reaction
  • Process 0.5 mg to 40 mg per preparation
  • Optimized to yield intact nuclei for CUT&RUN and CUT&Tag assays
 

Tissue Prep for NGS Assays Kit Contents

  • Tissue Lysis Buffer – NGS Assays, store at RT
  • 40 µm Strainer, store at RT
  • 1X Wash Buffer, store at 4°C
  • Protease Inhibitor Cocktail, store at -20°C
 

Tissue Prep for NGS Assays Kit Data

CUT&RUN Targeting Transcription Factors and Tissue Prep for NGS Assays
(Click image to enlarge)

Figure 1. Tissue Prep for NGS Assays is Optimized to Yield Nuclei for CUT&RUN Targeting Transcription Factors
10 mg mouse liver tissue were processed with Tissue Prep for NGS Assays. Isolated nuclei were obtained and 500,000 nuclei were used in duplicate CUT&RUN reactions targeting either YY1 or JunD. Libraries were generated with DNA Library Prep Kit for Illumina® Cat. No. 53220 and Dual Index Primers Set 1 Cat. No. 53221.

 

CUT&Tag-IT R-loop Assay Kit and Tissue Prep for NGS Assays(Click image to enlarge)

Figure 2. Tissue Prep for NGS Assays is Optimized to Yield Nuclei for CUT&Tag-IT R-loop Assay Kit
10 mg mouse liver tissue or mouse kidney tissue were processed with Tissue Prep for NGS Assays. Isolated nuclei were obtained and 500,000 nuclei were used in duplicate CUT&Tag-IT R-loop Assay reactions targeting DNA-RNA hybrids.

 

Tissue Prep for NGS Assays Kit Documents

 

 

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Name Format Cat No. Price  
Tissue Prep for NGS Assays 24 rxns 53185 $300 Add to Cart
Tissue Prep for NGS Assays Manual