Recombinant HDAC7 protein

Aliases: HD7; HD7A; HDAC7A
Catalog No: 31352 Format: 10 µg
Expressed In: Baculovirus Protein Species: Human

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Contents


This product has been discontinued:
Please refer to our alternative product Catalog No. 31535

10 µg recombinant HDAC7 supplied in a buffer of 40 mM Tris-HCl, pH 8.0, 270 mM NaCl, and 20% glycerol.

Background

HDAC7 (Histone Deacetylase 7) is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene. Positively regulates the transcriptional repressor activity of FOXP3 (PubMed:17360565).

Application Notes

Recombinant HDAC7 is suitable for use in histone deacetylase (HDAC) assays. It can also be used to study enzyme kinetics, inhibitor screening, and selectivity profiling.

Specific Activity: 17,824 pmol/min/μg.

Protein Details

The C-terminal part of HDAC7 (accession number AY302468) including the catalytic domain (amino acids 518-end) was expressed with an N-terminal GST-Tag (MW = 78 kDa) in a baculovirus expression system.

 
HDAC7 protein Coomassie gel

HDAC7 protein gel.
HDAC7 run on an SDS-PAGE gel and stained with Coomassie blue.

HDAC7 activity assay

HDAC7 activity assay.
Recombinant HDAC7 activity measured using a fluorescent HDAC assay.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.