Recombinant DNMT3L protein

Aliases: DNA Methyltransferase 3 Like,
Catalog No: 31414 Format: 20 µg $410 Buy
Catalog No: 31814 Format: 1 mg $3,500 Buy
Expressed In: Baculovirus Protein Species: Human

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Contents

Full length recombinant DNMT3L protein is supplied in 25 mM Tris pH 8.0, 300mM NaCl, 5% Glycerol and 0.04% Triton X-100, 1mM DTT. Please refer to product insert upon arrival for lot-specific concentration.

Background

DNMT3L (DNA (Cytosine-5-)-Methyltransferase 3-Like) is a nuclear protein with similarity to the DNMT family of proteins, but does not function as a DNA methyltransferase. It serves as an essential regulator of DNMT3A and DNMT3B, recruiting them to sites for deposition of de novo DNA methylation. DNMT3L is expressed in the presumptive gametes in mammalian embryos and is required for the establishment of genomic imprinting. DNMT3L has a PHD-like domain that allows binding to chromatin through interactions with the amino terminal tail of histone H3 that is devoid of lysine 4 methylation.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Full length recombinant DNMT3L protein (accession number NP_787063.1) was expressed in Sf9 cells and contains an N-terminal 6xHis-Tag with a molecular weight of 46.6 kDa. The recombinant protein is >80% pure by SDS-PAGE.

 
DNMT3L protein Coomassie gel

Recombinant DNMT3L protein gel.
Recombinant DNMT3L protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.