Recombinant SETD8 protein

Aliases: SET8, KMT5A, SET07, PR-Set7
Catalog No: 31427 Format: 20 µg $410 Buy
Expressed In: Baculovirus Protein Species: Human

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Contents

Full length recombinant SETD8 protein is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100 and 0.2 mg/ml 3× Flag peptide. Please refer to product insert upon arrival for lot-specific concentration.

Background

SETD8 (SET Domain Containing (Lysine Methyltransferase) 8) is Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins.It specifically catalyzes monomethylation of histone H4 lysine 20 (H4K20) which is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. H4K20me0 is also involved in DNA damage response. Besides H4K20, SETD8 monomethylates non-histone substrates including proliferating cell nuclear antigen (PCNA) and promotes carcinogenesis by deregulating PCNA expression. It also mediates monomethylation of p53/TP53 at 'Lys-382'and leads to repress p53/TP53-target genes.

Application Notes

Recombinant SETD8 protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.

Protein Details

Full length recombinant SETD8 protein (accession number NP_065115.3) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with a molecular weight of 42.2 kDa. The recombinant protein is >85% pure by SDS-PAGE.

 
SETD8 protein Coomassie gel

Recombinant SETD8 protein gel.
Recombinant SETD8 was run on an 8% SDS-PAGE gel and stained with Coomassie blue.

SETD8 protein HMT activity assay

Recombinant SETD8 protein activity assay.
Recombinant SETD8 activity titration was assessed using a Radiometry assay. Titration curves were generated to show signal response in the presence of histone peptide substrate at increasing SETD8 concentrations. This data was generated and kindly provided courtesy of ChemPartner.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.