Recombinant JARID1C / KDM5C protein

Aliases: Lysine Demethylase 5C, Jumonji, AT Rich Interactive Domain 1C (RBP2-Like)
Catalog No: 31433 Format: 20 µg $410 Buy
Catalog No: 31833 Format: 1 mg $3,800 Buy
Expressed In: Baculovirus Protein Species: Human

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Contents

Full length recombinant JARID1C / KDM5C protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

Background

Lysine (K)-specific demethylase 5C (KDM5C), also known as Jumonji, AT rich interactive domain 1C (JARID1C), is a member of the SMCY homolog family and encodes a protein with one ARID domain, one JmjC domain, one JmjN domain and two PHD-type zinc fingers. It is a histone demethylase that specifically demethylates lysine 4 of histone H3 (H3K4), thereby playing a central role in defining the histone code. KDM5B demethylates trimethylated and dimethylated, but not monomethylated H3K4. It does not demethylate histone H3K9, H3K27, H3K36, H3K79 or H4K20. KDM5C participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and CoREST pAb at neuron-restrictive silencer elements. Mutations in KDM5C have been associated with X-linked mental retardation.

Application Notes

Recombinant JARID1C / KDM5C is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Histone Demethylase Assay Conditions:50 mM HEPES pH 7.5, 0.02% Triton X100, 100 µM 2OG, 100 µM Ascorbate, 50 µM (NH4)2Fe(SO4)2·6H2O, 1 mM TCEP, 100 nM Recombinant JARID1C / KDM5C protein, and 3.3 µM H3K4me3 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.

Protein Details

Recombinant human JARID1C / KDM5C was expressed in a baculovirus expression system as the full length protein (accession number NP_004178.2) with an N-terminal FLAG tag. The molecular weight of the protein is 180 kDa.

References

This protein is described in the following references:
Xu J et al. (2008) PLoS One 3:e2553. doi: 10.1371/journal.pone.0002553
Iwase S et al. (2007) Cell 128:1077-1088

 
JARID1C / KDM5C Coomassie gel

Recombinant JARID1C / KDM5C protein gel.
JARID1C / KDM5C protein was run on a 8% SDS-PAGE gel and stained with Coomassie Blue.
MW: 180 kDa
Purity: > 87%

JARID1C / KDM5C activity assay

HTRF assay for JARID1C / KDM5C activity.
1 µM H3K4me3 peptide was incubated with JARID1C / KDM5C protein in reaction buffer for 1 hour at room temperature.H3K4me2 antibody was used to detect reaction product.

JARID1C / KDM5C activity assay

HTRF assay for JARID1C / KDM5C activity.
1 µM H3K4me3 peptide was incubated with JARID1C / KDM5C protein in reaction buffer for 1 hour at room temperature.H3K4me2 antibody was used to detect reaction product.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.