Recombinant ASH1L (2407-2579) protein
|Aliases: ASH1; KMT2H; ASH1L1|
|Catalog No: 31445||Format: 100 µg||$395||Buy|
|Catalog No: 31845||Format: 1 mg||$2,600||Buy|
Recombinant ASH1L (2407-2579) protein was expressed in E. coli cells and is supplied in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, and 10% glycerol. Please refer to product insert upon arrival for lot-specific concentration.
ASH1L is a member of the Trithorax group of transcriptional activators. The protein contains four AT hooks, a SET domain, a PHD-finger motif, and a bromodomain. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. ASH1L is localized to the nucleus and also to cell-cell tight junctions where it is hypothesized to function in adhesion-mediated signaling. ASH1L functions in methylation of H3K4 and regulation of expression of HOX genes and may possess methyltransferase activity directed at H3K36. ASH1L has also been implicated in facioscapulohumeral muscular dystrophy.
Recombinant ASH1L (2407-2579) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
The peptide corresponding to amino acids 2407-2579 that contains the bromodomain sequences of ASH1L (accession number NP_060959.2) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with an observed molecular weight of 23.2 kDa.
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.