Recombinant KAT7 protein

Aliases: Lysine Acetyltransferase 7, HBO1, HBOA, MYST2, Histone Acetyltransferase Binding To ORC1
Catalog No: 31489 Format: 20 µg $410 Buy
Expressed In: Baculovirus Protein Species: Human

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Contents

Full length recombinant KAT7 protein is supplied in 25 mM Hepes pH 7.5, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2 mM TCEP, 0.2 mg/ml 3× Flag peptide. Please refer to product insert upon arrival for lot-specific concentration.

Background

KAT7 (K(Lysine) Acetyltransferase 7) is a MYST-family histone acetyltransferase (HAT) that forms a complex with ING4/5, JADE1/2/3, and Eaf6, which is found enriched on coding regions of genes, supporting a role in transcriptional elongation. In addition, KAT7 is thought to participate in events controlling the initiation of DNA replication.

Application Notes

Recombinant KAT7 protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.

Protein Details

Full length recombinant KAT7 protein (accession number NP_001186084.1) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with a molecular weight of 67.2 kDa. The recombinant protein is >85% pure by SDS-PAGE.

 
KAT7 protein Coomassie gel

Recombinant KAT7 protein gel.
Recombinant KAT7 was run on an 8% SDS-PAGE gel and stained with Coomassie blue.

KAT7 protein activity assay

Recombinant KAT7 protein activity assay
Recombinant KAT7 activity titration was assessed using a Radiometry assay. Titration curves were generated to show signal response in the presence of histone peptide substrate at increasing KAT7 concentrations. This data was generated and kindly provided courtesy of ChemPartner.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.