Recombinant Histone H2A (Human)

Catalog No: 31490 Format: 100 µg $110 Buy
Catalog No: 31890 Format: 1 mg $480 Buy
Expressed In: E. coli Protein Species: Human

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Contents

Full length recombinant Histone H2A (Human) was expressed in E.coli and is supplied in 25 mM Tris pH 8.0, 150 mM NaCl, 5% Glycerol. Please refer to product insert upon arrival for lot-specific concentration.

Background

Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points.

Application Notes

Recombinant Histone H2A (Human) is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Protein Details

Recombinant Histone H2A (Human) (assession number: NP_003503.1) was expressed in E. coli cells. This protein does not contain any tag with a molecular weight of 14.1 kDa. The recombinant histone is >90% pure by SDS-PAGE. The recombinant protein is >85% pure by SDS-PAGE.

Protein Sequence: SGRGKQGGKA RAKAKSRSSR AGLQFPVGRV HRLLRKGNYA ERVGAGAPVY LAAVLEYLTA EILELAGNAA RDNKKTRIIP RHLQLAIRND EELNKLLGRV TIAQGGVLPN IQAVLLPKKT ESHHKAKGK

 
Histone H2A protein Coomassie gel

Recombinant Histone H2A (Human) protein gel.
Histone H2A protein was run on a 15% SDS-PAGE gel and stained with Coomassie blue.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.