Recombinant Histone H4, His-Tag (Human)

Catalog No: 31493 Format: 100 µg $110 Buy
Catalog No: 31893 Format: 1 mg $480 Buy
Expressed In: E. coli Protein Species: Human

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Contents

Full length recombinant Histone H4, His-Tag (Human) is supplied in 25 mM Tris pH 7.4, 150 mM NaCl, 5% Glycerol. Please refer to product insert upon arrival for lot-specific concentration.

Background

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points.

Application Notes

Recombinant Histone H4, His-Tag (Human) is suitable for use as substrate for histone modification enzymes, or to generate chromatin in vitro.

Protein Details

Recombinant Histone H4, His-Tag (Human) (assession number: NP_003539.1) was expressed in E. coli cells and contains a C-terminal 6×His-Tag with a molecular weight of 12.19 kDa. The recombinant histone H4 is >85% pure by SDS-PAGE.
Protein Sequence (Tag sequence in italics): SGRGKGGKGL GKGGAKRHRK VLRDNIQGIT KPAIRRLARR GGVKRISGLI YEETRGVLKV FLENVIRDAV TYTEHAKRKT VTAMDVVYAL KRQGRTLYGF GGLEHHHHHH

 
Recombinant Histone H4, His-Tag Coomassie gel

Recombinant Histone H4, His-Tag (Human) protein gel.
Recombinant Histone H4, His-Tag run on a 12% SDS-PAGE gel and stained with Coomassie blue.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.