Recombinant HDAC2 protein
|Aliases: Histone Deacteylase 2, HD2, RPD3, YAF1|
|Catalog No: 31505||Format: 50 µg||$435||Buy|
|Catalog No: 31909||Format: 1 mg||$3,660||Buy|
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Expressed In: Baculovirus Protein Species: Human
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
HDAC2 (Histone Deacetylase 2, also designated mammalian RPD3) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.
In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and HDAC10), class III (SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6 and SIRT-7) and class IV (HDAC11).
HDAC2 is associated with many different proteins as YY1 (a mammalian zinc-finger transcription factor). HDAC2 also forms transcriptional repressor complexes containing, among others, HDAC1 or RBBP4. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both Histone H3 and Histone H4 in free histones or nucleosome substrate.
Recombinant HDAC2 is suitable for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active Motif offers a variety of protein screening tools including HDAC Assay Kits.
HDAC Activity Assay Conditions: 3 μM Histone H3K9ac (1-21aa) peptide was incubated with HDAC2 protein in reaction buffer including 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM MgCl2, 1 mM KCl and 0.1 mg/ml BSA for 30 min at 37°C. HTRF assay was used for activity detection.
Active Motif has a collection of small-molecule epigenetic modulators that target HDACs - click here to view the list.
Recombinant human HDAC2 was expressed in Sf9 cells as the full length protein (accession number NP_001518.3) with a C-terminal FLAG-Tag. The molecular weight of the protein is 60 kDa. The recombinant protein is >80% pure by SDS-PAGE.
This product was used in the following publications:
Cell Rep. (2018). 24(13): 3488-3502. PMID: 30257210. Histone Deacetylase (HDAC) Assay.
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.