Recombinant AKT1 protein
|Aliases: AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA|
|Catalog No: 31511||Format: 10 µg||$425||Buy|
Expressed In: Baculovirus Protein Species: Human
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
AKT1 is a serine-threonine protein kinase that is activated by Platelet-Derived Growth Factor (PDGF) and PI 3-kinase signaling pathways. AKT has been shown to be a critical mediator of survival and apoptosis signaling events. Activation of AKT1 involves (and requires) phosphorylation of serine 473. AKT is also referred to as Protein Kinase B (PKB) and RAC.
Recombinant AKT1 is suitable for kinase assays. The molecular weight of the fusion protein is ~ 81 kDa.
Specific activity: 55 pmol/μg×min ATP-KM: 5.0 μM
Recombinant AKT1 (accession number NP_005154.2) activated with PDK1, was expressed in Sf9 cells is an N-terminal GST-HIS6 fusion protein with a 3C cleavage site.
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.