Recombinant AKT1 protein

Aliases: AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA
Catalog No: 31511 Format: 10 µg $410 Buy
Expressed In: Baculovirus Protein Species: Human

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Contents

Full length recombinant AKT1 protein expressed in Sf9 cells and supplied in 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

Background

AKT1 is a serine-threonine protein kinase that is activated by Platelet-Derived Growth Factor (PDGF) and PI 3-kinase signaling pathways. AKT has been shown to be a critical mediator of survival and apoptosis signaling events. Activation of AKT1 involves (and requires) phosphorylation of serine 473. AKT is also referred to as Protein Kinase B (PKB) and RAC.

Application Notes

Recombinant AKT1 is suitable for kinase assays. The molecular weight of the fusion protein is ~ 81 kDa.

Specific activity: 55 pmol/μg×min ATP-KM: 5.0 μM

Protein Details

Recombinant AKT1 (accession number NP_005154.2) activated with PDK1, was expressed in Sf9 cells is an N-terminal GST-HIS6 fusion protein with a 3C cleavage site.

 
AKT1 protein Coomassie gel

Recombinant AKT1 protein gel.
AKT1 protein was run on an SDS-PAGE gel and stained with Coomassie blue.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.