Recombinant AGO3 protein
|Aliases: Argonaute 3, RISC Catalytic Component, Eukaryotic Translation Factor 2C, EIF2C3, HAgo3, Argonaute3, EIF-2C, EIF2C|
|Catalog No: 31523||Format: 20 µg||$420||Buy|
|Catalog No: 31923||Format: 1 mg||$3,550||Buy|
Recombinant full length AGO3 protein was expressed in Sf9 cells and is provided in 25 mM Tris pH 8.0, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mg/ml 3X Flag peptide. Please refer to product insert upon arrival for lot-specific concentration.
AGO3 (argonaute RISC catalytic component 3, also known as EIF2C3) is a member of the Argonaute family of proteins which play a role in RNA interference. The Argonaute family proteins (AGO1, AGO2, AGO3 and AGO4) are involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. RISC uses the siRNA or miRNA as a template for recognizing complementary mRNA. When it recognizes a complementary strand, it activates AGO2 to cleaves the mRNA. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. In some cases, RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3’ untranslated region (3’-UTR). AGO3 lacks endonuclease activity and does not appear to cleave target mRNAs. AGO3 contain a PAZ and a PIWI domain.
Recombinant AGO3 is suitable for use in the study of miRNA screening, RNAi research and selectivity profiling. This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Recombinant human AGO3 was expressed in a baculovirus expression system as the full length protein (accession number NP_079128.2) with an N-terminal FLAG-Tag. The molecular weight of the protein is 100.4 kDa. The AGO3 protein is >75% pure by SDS-PAGE.
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.