Recombinant HDAC4 (627-1084) protein

Aliases: Histone Deacetylase 4, HD4, Histone Deactylase A, HDAC-4, HDAC-A
Catalog No: 31527 Format: 20 µg $410 Buy
Catalog No: 31927 Format: 1 mg $3,550 Buy

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Expressed In: Baculovirus Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

HDAC4 (Histone Deacetylase 4) is a member of the class IIa mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.
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In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). Unlike other deacetylases, HDAC4 shuttles between the nucleus and cytoplasm and serves as a nuclear co-repressor that regulates bone and muscle development. HDAC4 interacts with the myocyte enhancer factors Mef2a, Mef2c and Mef2d. It also forms part of a multi-protein complex with RbAp48 and HDAC3. HDAC4 is ubiquitous.

Application Notes

Recombinant HDAC4 protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

HDAC Activity Assay Conditions: Assay was performed using HDAC-Glo Class IIa Assay from Promega. 3.5 µM substrate was incubated with HDAC4 proteins and 1/20,000 developer reagent at room temperature, then luminescence was detected after incubation for 20 min

Active Motif has a collection of small-molecule epigenetic modulators that target HDACs - click here to view the list.

Protein Details

Recombinant HDAC4 (627-1084) protein that includes amino acids 627-1084 of human HDAC4 protein (accession number NP_006028.2) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with a molecular weight of 52.9 kDa. The purity of HDAC4 protein is >90% by SDS-PAGE.

 

Recombinant HDAC4 (627-1084) protein gel. HDAC4 (627-1084) protein was run on an 8% SDS- PAGE gel and stained with Coomassie Blue.

HDAC-Glo Class IIa Assay for HDAC4 activity. Assay was performed using HDAC-Glo Class IIa Assay from Promega. 3.5 µM substrate was incubated with HDAC4 proteins and 1/20,000 developer reagent at room temperature, then luminescence was detected after incubation for 20 min.

HDAC-Glo Class IIa Assay for HDAC4 activity. Assay was performed using HDAC-Glo Class IIa Assay from Promega. 3.5 µM substrate was incubated with HDAC4 proteins and 1/20,000 developer reagent at room temperature, then luminescence was detected after incubation for 20 min.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.