Recombinant NONO protein

Aliases: Non-POU Domain Containing, Octamer-Binding, NMT55, P54NRB , Nuclear RNA-Binding Protein,
Catalog No: 31539 Format: 20 µg $410 Buy
Expressed In: Baculovirus Protein Species: Human

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Contents

Recombinant full length NONO protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

Background

NONO (non-POU domain containing, octamer-binding, p54(nrb), NRB54, NMT55) is a DNA- and RNA binding protein and involved in several nuclear processes. NONO binds the conventional octamer sequence in double-stranded DNA. It also binds single-stranded DNA and RNA at a site independent of the duplex site. This gene also encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Recombinant human NONO was expressed in a baculovirus expression system as the full length protein (accession number NP_031389.3) with an N-terminal FLAG-Tag. The molecular weight of the protein is 57.2 kDa. The NONO protein is >90% pure by SDS-PAGE.

 
Recombinant NONO protein gel.

Recombinant NONO protein gel.NONO protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.