Recombinant DNMT3B (212-358) protein

Aliases: DNA Methyltransferase 3 Beta, ICF1, EC 2.1.1.37
Catalog No: 31542 Format: 100 µg $410 Buy
Catalog No: 31942 Format: 1 mg $2,900 Buy
Expressed In: E. coli Protein Species: Human

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Contents

Recombinant DNMT3B (212-358) was expressed in E. colicells and is supplied in 25 mM Tris pH 7.4, 150 mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

Background

DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 Beta)protein is a de novo DNA methyltransferase. It is involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and control of gene expression. DNA methylation is generally associated with transcriptional repression.

Trimethylation of Lys36 on histone H3 tail (H3K36me3) is associated with DNA methylation and elongation phase of transcription. PWWP domain of DNMT3B reads this epigenetic mark to guide DNA methylation.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

The peptide corresponding to amino acids 212 - 358 that contains the PWWP sequences of DNMT3B (accession number NP_715640.2) was expressed in E. coli and contains an N-terminal GST-Tag with an observed molecular weight of 42.4 kDa. The recombinant protein is >90% pure by SDS-PAGE.

 
Recombinant DNMT3B (212-358) gel.

Recombinant DNMT3B (212-358) gel.
DNMT3B (212-358) was run on a 12% SDS-PAGE gel and stained with Coomassie Blue.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.