Recombinant METTL14 protein

Catalog No: 31568 Format: 20 µg $415 Buy
Expressed In: Baculovirus Protein Species: Human

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Contents

Recombinant METTL14 protein is supplied at a concentration of 0.2 µg/µl in 25 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP.

Background

N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messager RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A also takes place in other RNA molecules, such as primary miRNA (pri-miRNAs).
METTL14 (methyltransferase-like 14) forms a stable N6-methyltransferase heterodimer complex with METTL3, which catalyzes the generation of m6A modification on mammalian nuclear RNAs. METTL3 is the catalytically active subunit, while METTL14 plays a structural role critical for substrate recognition.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Recombinant METTL14 protein was expressed in a Baculovirus expression system as the full length protein (accession number NP_066012.1) with an N-terminal FLAG-Tag. The molecular weight of METTL14 is 53.3 kDa.

 
Recombinant METTL14 protein gel.

Recombinant METTL14 protein gel
Recombinant METTL14 was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.
MW: 53.3 kDa
Purity: > 75%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.