Histone H2A/H4S1ph antibody (pAb)

Aliases: Histone H1A/H4 phosphoserine-1
Catalog No: 39115 Format: 200 µl $435 Buy
Catalog No: 39116 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
14 and 8 kDa
Reactivity:
Human, Other (Wide Range)

Applications

Western Blot Validated Dot Blot Validated

Application Notes


Validated Applications:
WB: 1:1,000 - 1:50,000 dilution

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot. 

Immunogen

This Histone H2A/H4 phospho Ser1 antibody was raised against a peptide including phospho-serine 1 of human histone H2A.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

View our Guide to Histone Modifications and Biological Function.

References

 
Histone H2A/H4S1ph antibody (pAb) tested by Western blot.

Histone H2A/H4 phospho Ser1 pAb tested by Western blot.
HeLa acid extract (5 µg per lane) was probed with Catalog No. 39115 (1:30,000 dilution).
    Lane 1: No treatment.
    Lane 2: Cells treated with colcemid.

Histone H2A/H4S1ph antibody (pAb) tested by dot blot analysis.

Histone H2A/H4 phospho Ser1 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H2A/H4 phospho Ser1 pAb for phospho Ser1 of Histone H2A/H4. Modified and unmodified peptides were spotted onto PVDF and probed with the antibody at a 1:10,000 dilution. The amount of peptide spotted (in picomoles) is indicated next to each row.
    Lane 1: Peptide phosphorylated at Ser1.
    Lane 2: Unmodified Ser1 peptide.

Background

Histones H2A and H4 are core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

The first five amino acids of histone H2A and histone H4 are identical in metazoans and include an amino-terminal serine residue. Histone H2A phosphorylation at serine 1 is enriched during mitosis, while H4 phosphorylation increases subsequent to DNA double-strand breaks.

Positive Control

Active Motif's HeLa acid extract (Paclitaxel treated) (Catalog No. 36201) can be used as a positive control for Histone H2A/H4 phospho Ser1 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting