Histone H3T3ph antibody (pAb)

Catalog No: 39153 Format: 200 µl $435 Buy
Catalog No: 39154 Format: 10 µl $105 Buy

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Antibody Type:
Molecular Weight:
17 kDa
Human, Other (Wide Range)


Western Blot Validated Immunofluorescence Validated Dot Blot Validated Immunocytochemistry Validated

Application Notes

Validated Applications:
WB: 1:500 - 1:2,000 dilution

Published Applications:

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.


This Histone H3 phospho Thr3 antibody was raised against a peptide including phospho-threonine 3 of histone H3.


Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

View our Guide to Histone Modifications and Biological Function.


Histone H3T3ph antibody (pAb) tested by Western blot.

Histone H3 phospho Thr3 pAb tested by Western blot.
Western blot probed with Histone H3 phospho Thr3 pAb (1:500 dilution).
    Lane 1: 20 µg acid extract of HeLa cells.
    Lane 2: 20 µg acid extract of HeLa cells treated with colcemid.

Histone H3T3ph antibody (pAb) tested by dot blot analysis.

Histone H3 phospho Thr3 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 phospho Thr3 pAb for phospho-Thr3 of histone H3. Decreasing amounts of peptides corresponding to regions around major sites of histone H3 threonine phosphorylation were spotted onto PVDF and probed with the antibody at a dilution of 1:2,000.
    Lane 1: Peptide phosphorylated at threonine 3.
    Lane 2: Unmodified threonine 3 peptide.
    Lane 3: Peptide phosphorylated at threonine 11.
    Lane 4: Unmodified threonine 11 peptide.
    Lane 5: Peptide phosphorylated at threonine 45.
    Lane 6: Unmodified threonine 45 peptide.


Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

In vivo, phosphorylation of histone H3 at threonine 3 begins at early prophase in the vicinity of the nuclear envelope, spreads to the pericentromeric chromatin during prometaphase and is fully reversed by late anaphase, during mitosis.

Positive Control

Active Motif's HeLa acid extract (Paclitaxel treated) (Catalog No. 36201) can be used as a positive control for Histone H3 phospho Thr3 antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3T3ph antibody (pAb).


Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.


This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting