Ikaros antibody (pAb)

Aliases: IKZF1, LyF-1, hlk-1, Ikaros, Zfpn1a1, Znfn1a1
Catalog No: 39291 Format: 200 µl $435 Buy
Catalog No: 39292 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
62 kDa
Reactivity:
Mouse

Applications

ChIP Validated Western Blot Validated Electrophoretic Mobility Shift Assay Validated

Application Notes


Validated Applications:
WB: 1:500 dilution

Published Applications:
ChIP
EMSA

Ikaros may be present as multiple protein isoforms due to alternative splicing. Commonly, the two largest isoforms are detected as seen in the example Western blot image.

Immunogen

This Ikaros antibody was raised against a recombinant protein corresponding to the N-terminal half of mouse Ikaros.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
Ikaros antibody (pAb) tested by Western blot.

Ikaros pAb tested by Western blot.
Detection of Ikaros by Western blot analysis. Ikaros was detected using a 1:1,000 dilution of Ikaros pAb with 20 µg mouse spleen tissue extract.

Background

Ikaros is a transcription factor that plays a critical role in the control of lymphohematopoiesis and immune regulation. All Ikaros isoforms share a common C-terminal domain that contains a transcription activation motif and two zinc finger motifs required for hetero- and homo-dimerization among the Ikaros isoforms, and for interactions with other proteins. Ikaros is abundantly expressed in pituitary mammosomatotrophs, where it deacetylates Histone H3 sites on the proximal growth hormone (GH) promoter to silence gene expression.

Positive Control

Whole-cell extracts from mouse spleen can be used as a positive control for Ikaros antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Ikaros antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting