Nap1 antibody (pAb)

Catalog No: 39577 Format: 100 µl $435 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
51 kDa
Reactivity:
Drosophila

Applications

Western Blot Validated Immunofluorescence Validated

Application Notes


Validated Applications:
WB: 1:1,000 - 1:5,000 dilution

Published Applications:
IF
WB

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Immunogen

This Nap1 antibody was raised against a His-Tagged full-length protein corresponding to Drosophila Nap1.

Buffer

PBS containing 0.1% sodium azide, 0.2 mg/ml BSA and 35% glycerol. Sodium azide is highly toxic.

References

 
Nap1 antibody (pAb) tested by Western blot.

Nap1 pAb tested by Western blot.
Schneider's Drosophila L2 nuclear extract (10 µg) was probed with Nap1 pAb (1:1,000 dilution).

Background

Nap1 (Nucleosome assembly protein 1) is a member of the nucleosome assembly protein (NAP) family. Nap1 serves as a chaperone for Histone H2A-Histone H2B dimers, and participates in DNA replication and may play a role in modulating chromatin formation and contribute to the regulation of cell proliferation. Nap1 binds the HIV Rev protein, stimulating its RNA export function, while causing it to localize away from the nucleolus.

Positive Control

Active Motif's Schneider's Drosophila L2 nuclear extract (Catalog No. 36087) can be used as a positive control for Nap1 antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Nap1 antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting