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Ets-1 antibody (pAb)
| RRID: AB_2793266 |
| Aliases: ETS1, EWSR2 |
| Catalog No: 39580 | Format: 100 µl | $510 | Add to Cart |
| Catalog No: 39581 | Format: 10 µl | $125 | Add to Cart |
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Applications
Application Notes
Applications Validated by Active Motif:
ChIP-Seq: 4 µg (7 µl) per ChIP
WB: 1:500 - 1:1,000 dilution
CUT&RUN: 1 µl per 50 µl reaction
Immunogen
This Ets-1 antibody was raised against a peptide corresponding to the C-terminal half of mouse Ets-1.
Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
ETS-1 antibody (pAb) tested by ChIP-Seq.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from human CD4+ T cell chromatin and 4 µg ETS-1 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 7.9 million sequence tags were mapped to identify ETS-1 binding sites in a region of human chromosome 19.
Ets-1 pAb tested by Western blot.
Detection of Ets-1 by Western blot. The analysis was performed using CCRF-CEM nuclear extract and Ets-1 pAb at a 1:500 dilution.
Ets-1 antibody (pAb) tested by CUT&RUN
CUT&RUN was performed using 500,000 K562 nuclei and sequenced using 38 base-pair, paired-end reads on the Illumina NovaSeq. Data was collected from 49 million reads, and Ets-1 data is shown for Chromosome 19.
Background
Ets-1 transcription factor is a member of the Ets family of proteins that share a unique binding domain. Ets1 (E26 transformation-specific sequence-1) is a proto-oncogene whose DNA binding activity is controlled by kinases and other transcription factors. Ets 1 has been associated with regulation of cell growth, differentiation and apoptosis.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Ets-1 antibody (pAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
