PXR antibody (pAb)

Aliases: Nuclear Receptor Subfamily 1 Group I Member 2, Orphan Nuclear Receptor PXR, Pregnane X Receptor, Orphan Nuclear Receptor PAR1
Catalog No: 39589 Format: 200 µl $435 Buy
Catalog No: 39590 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
54 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

Immunogen

This PXR antibody was raised against a peptide containing amino acid residues 57-71 of human PXR.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
PXR antibody (pAb) tested by Western blot.

PXR pAb tested by Western blot.
Hep G2 nuclear extract (20 µg per lane) was probed with PXR pAb at a dilution of 1:1,000.

Background

PXR (NR1I2, Pregnane X receptor, nuclear receptor subfamily 1 group I member 2) belongs to a family of transcription factors characterized by a ligand-binding domain and a DNA-binding domain. PXR is an orphan receptor that regulates transcription of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR (RXR-α and RXR-β). PXR is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin.

Positive Control

Active Motif's Hep G2 nuclear extract (Catalog No. 36011) can be used as a positive control for PXR antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting