Uhrf1 antibody (pAb)

RRID: AB_2793280
Aliases: Np95, hNP95, ICBP90, RNF106, hUHRF1, huNp95
Catalog No: 39625 Format: 200 µl $460 Buy
Catalog No: 39626 Format: 10 µl $115 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
96 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 1:500 - 1:1,000 dilution

Immunogen

This Uhrf1 antibody was raised against a peptide corresponding to the N-terminal half of human Uhrf1.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

Uhrf1 pAb tested by Western blot. HEK293 nuclear extract (20 µg per lane) was probed with Uhrf1 pAb (1:500).

Background

Uhrf1 (ubiquitin-like, containing PHD and RING finger domains 1, also known as ICBP90, RNF106 or NP95) is a transcription and cell cycle regulator. It functions as a putative E3 ubiquitin-protein ligase. Uhrf1 is important for G1/S transition of the cell cycle. It is also believed that Uhrf1 may help recruit the DNA methyltransferase, DNMT1, to hemimethylated DNA and assist in the stabilization of DNMT1 with chromatin.

Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for Uhrf1 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting