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Histone H2A.X antibody (pAb)

Catalog No: 39689 Format: 100 µl $425 Buy Now
Catalog No: 39690 Format: 10 µl $99 Buy Now

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Antibody Type:Polyclonal
Purification:Affinity Purified
Molecular Weight:15 kDa


ChIP Validated Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for Histone H2A.X antibody (pAb) (H2AX)Application Notes

Validated Applications:
ICC/IF: 1:500 - 1:1,000 dilution
WB: 1:500 - 1:2,000 dilution

Published Applications:

for Histone H2A.X antibody (pAb) (H2AX)Immunogen

This Histone H2AX antibody was raised against a peptide derived from the C-terminus of human histone H2AX.

for Histone H2A.X antibody (pAb) (H2AX)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Histone H2A.X antibody (pAb) (H2AX)References

Histone H2A.X antibody (pAb) tested by immunofluorescence.

Histone H2AX antibody tested by immunofluorescence.
Detection of Histone H2AX by immunofluorescence. HeLa cells were stained with Histone H2AX antibody at a dilution of 1:1,000. Top panel: Histone H2AX antibody staining. Middle panel: DAPI. Bottom panel: merge.

Histone H2A.X antibody (pAb) tested by Western blot.

Histone H2AX pAb tested by Western blot.
Western blot probed with Histone H2AX pAb at a dilution of 1:2,000. Lane 1: Recombinant human histone H2AX (100 ng). Lane 2: Mouse embryonic fibroblast (MEF) cell wild type nuclear lysate. Lane 3: MEF cell nuclear extract derived from H2AX -/- nulls.

for Histone H2A.X antibody (pAb) (H2AX)Background

Histone H2AX (H2AX, H2A histone family member X) replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries that require DNA as a template. Histones thereby play a central role in transcriptional regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called the histone code, and nucleosome remodeling. Histone H2AX is required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation, and for efficient repair of DNA double-strand breaks (DSBs), specifically when modified by C-terminal phosphorylation.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H2A.X antibody (pAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H2A.X antibody (pAb).

for Histone H2A.X antibody (pAb) (H2AX)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H2A.X antibody (pAb) (H2AX)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

Histone H2A.X antibody (pAb)

Data Thumbnails

Histone H2A.X antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of Histone H2A.X pAb.
(Click image to enlarge and see details.)

Histone H2A.X antibody (pAb) tested by Western blot.

Western blot of Histone H2A.X pAb.
(Click image to enlarge and see details.)