Oct-4 antibody (pAb)
|Aliases: POU5F1, OCT3, OTF3, OTF4, OTF-3, Oct-3|
|Catalog No: 39811||Format: 100 µl||$490||Buy|
|Catalog No: 39812||Format: 10 µl||$120||Buy|
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Applications Validated by Active Motif:
ChIP: 10 µl per ChIP
ChIP-Seq: 10 µl each
ICC/IF: 1:200 dilution
WB: 1:1,000 - 1:5,000 dilution
ChIP-Seq validation was performed by Active Motif's Epigenetics Services; the complete data set is available in the UCSC Genome Browser by clicking here.
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
This Oct-4 antibody was raised against a peptide derived from human Oct-4.
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Oct-4 (Octamer binding protein-4, POU5F1) is a member of the POU family of transcriptional activators. Oct-4 is critical for early embryogenesis and required for embryonic stem cell pluripotency. Oct-4 is expressed at high levels in undifferentiated cells and ectopic expression of Oct-4 (and several other transcription factors) can induce pluripotency in differentiated cells.
What are the Epigenetic Determinants of Stem Cell Identity?
Active Motif's P19 nuclear extract (Catalog No. 36074) can be used as a positive control for Oct-4 antibody.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Oct-4 antibody (pAb).
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting