Sox2 antibody (pAb)

RRID: AB_2750954
Aliases: ANOP3, MCOPS3
Catalog No: 39843 Format: 100 µl $455 Buy
Catalog No: 39844 Format: 10 µl $110 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
42 kDa
Reactivity:
Human, Mouse

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes


Validated Applications:
ChIP: 10 µl per ChIP
ChIP-Seq: 10 µl each
ICC/IF: 1:200 dilution
WB: 1:500 - 1:1,000 dilution

ChIP-Seq validation was performed by Active Motif's Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here.

Published Applications:
ChIP-qPCR
ICC/IF

Immunogen

This Sox2 antibody was raised against a peptide within the C-terminal region of human Sox2.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 

Sox2 antibody (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from undifferentiated hESC cells and 7 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 10 million sequence tags were mapped to identify Sox2 binding sites. The image shows binding across a region of chromosome 7. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

Sox2 antibody (pAb) tested by Immunofluorescence.
Mouse embryonic stem cells (mESCs) grown on mouse embryonic fibroblast feeder cells (MEFs) were fixed with 4% paraformaldehyde for 10 minutes at room temperature. Cells were then permeabilized and blocked by incubating with Blocking Solution containing 5% serum/0.1% Triton X-100 in D-PBS for 2 hours at room temperature. Cells were then incubated with Sox2 antibody (Catalog No. 39843, red) at 1:200 dilution overnight at 4°C, washed with D-PBS, and incubated for 2 hours at room temperature with goat anti-mouse Cy3 secondary antibody at 1:250 dilution. Cells were visualized using a Zeiss fluorescent microscope at 20X magnification. Images show that Sox2 antibody specifically stains mESC colonies and does not stain MEFs. Absence of Sox2 staining in a subset of cells within the colonies suggests differentiation. Scale bars, 100 μm.

Sox2 antibody (pAb) tested by Western blot.
Nuclear extract of P19 cells (20 µg per lane) probed with Sox2 antibody (pAb) (1:500 dilution).

Background

Sox2 (SRY related HMG BOX gene 2) is a DNA binding transcription factor and a member of the SOX family of proteins. SOX proteins have an HMG box that binds DNA. Sox2 forms a complex with Oct-4 and controls the expression of a number of genes involved in embryonic. Sox2 is critical for early embryogenesis and for embryonic stem cell pluripotency and thus can serve as a stem cell marker. Overexpression of Sox2 (along with Oct-4, KLF4 and c-Myc) can transform mouse fibroblasts into a state resembling embryonic stem cells (ES cells), referred to as Induced Pluripotency. Defects in Sox2 are the cause of microphthalmia syndromic type 3.

Positive Control

Active Motif's P19 Nuclear Extract (Catalog No. 35074) can be used as a positive control for Sox2 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Sox2 antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting