NCoA4 / ELE1 antibody (pAb)

Aliases: RFG, PTC3, ARA70
Catalog No: 39895 Format: 100 µl $435 Buy
Catalog No: 39896 Format: 10 µl $105 Buy

Request a quote for a bulk order Bulk Order Button


Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
85 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

Immunogen

This NCoA4 / ELE1 antibody was raised against a peptide derived from the C-terminal region of human NCoA4.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
NCoA4 / ELE1 antibody (pAb) tested by Western blot.

NCoA4 / ELE1 antibody (pAb) tested by Western blot.
Nuclear extract (20 µg) of LNCaP cells probed with NCoA4 / ELE1 antibody at a dilution of 1:2,000.

Background

NCoA4 (Nuclear receptor co-activator 4; also known as ELE1 and ARA70) is a transcriptional co-activator that enhances the transcriptional activity of several nuclear receptors, including androgen receptor and peroxisome proliferator-activated receptor (PPAR) gamma. It also interacts with the androgen receptor and the retinoid X receptor (RXR) in a ligand-dependent manner.

Positive Control

Active Motif's LNCaP nuclear extract (Clone FGC)(Catalog No. 36051) can be used as a positive control for NCoA4 / ELE1 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting