RAR-α antibody (mAb)

Clone: 9α-9A6
Aliases: Retinoic Acid Receptor Alpha, RARA, RAR, NR1B1
Catalog No: 39971 Format: 100 µg $435 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1k
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
52 kDa
Reactivity:
Human, Mouse

Applications

Western Blot Validated Immunohistochemistry Validated Electrophoretic Mobility Shift Assay Validated

Application Notes


Published Applications:
EMSA
IHC
WB
See references for more information. Individual optimization may be required.

Immunogen

This RAR-a antibody was raised against a recombinant protein corresponding to amino acids 420-443 (the F region) of human RARa.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

Background

Retinoic acid receptors (RAR) are nuclear receptors that are activated by both all-trans retinoic acid and 9-cis retinoic acid. There are three retinoic acid receptors (RAR), RAR-α, RAR-β and RAR-γ. RAR-α (Nuclear receptor subfamily 1 group B member 1, NR1B1) interacts with NCoA3, NCoA6, NCoA7 and MLL to activate transcription of target genes. Chromosomal aberrations of RAR-α are sometimes involved in Promyelocytic leukemia.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting