MyoD antibody (mAb)

Clone: 5F11
Aliases: MYOD1, PUM, MYF3, bHLHc1
Catalog No: 39991 Format: 100 µg $435 Buy
Catalog No: 39992 Format: 10 µg $105 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG2a
Purification:
Protein G Chromatography
Host:
Rat
Molecular Weight:
40 kDa
Reactivity:
Human, Mouse

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes


Published Applications:
ICC/IF
WB
See references for more information. Individual optimization may be required.

Immunogen

This MyoD antibody was raised against a recombinant protein corresponding to full-length mouse MyoD.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

Background

MyoD (Myogenic differentiation factor 1, Myf-3), is a transcription factor belonging to the myogenic factors family that contains a basic helix-loop-helix (bHLH) DNA-binding domain. MyoD is the central regulator in the signaling pathways that regulate muscle development.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with MyoD antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting