HDAC1 antibody (pAb)

RRID: AB_2614948
Aliases: HD1, RPD3, GON-10, RPD3L1
Catalog No: 40967 Format: 100 µg $470 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
60 kDa
Reactivity:
Human, Mouse, Rat

Applications

CUT&RUN Validated ChIP Validated ChIP-Seq Validated Western Blot Validated

Application Notes

Applications Validated by Active Motif:
ChIP: 4 µg per ChIP
ChIP-Seq & ChIP-chip: 4 µg each
WB: 1 - 2 µg/ml dilution

For optimal results in Western blotting, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

For HDAC1, we also offer AbFlex® HDAC1 Recombinant Antibody (rAb). For details, see Catalog No. 91215.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP-qPCR
CUT&RUN
IP
WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This HDAC1 antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 1-5, 433-448 and 467-482 of human HDAC1.

Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

 

HDAC1 pAb tested by ChIP-Chip.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of mouse hippocampus chromatin and 4 ug of HDAC1 antibody. ChIP DNA was amplified by WGA, labeled and hybridized to a mouse tiling array. The left side of the image shows HDAC1 binding at multiple promoters. The right side of the image shows HDAC1 binding spread broadly across the Tcf4 gene.

HDAC1 pAb tested by ChIP-qPCR.
Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 20 µg of rat brain chromatin and 4 µg of HDAC1 antibody. ChIP DNA was used in qPCR with the negative control primer pairs and primers that amplify the Actb promoter. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

HDAC1 pAb tested by Western blot.
Detection of HDAC1 by Western blot analysis. 293 nuclear extract was probed with HDAC1 pAb. A protein band of approximate molecular weight of 60 kDa was detected.

Background

HDAC1 (Histone Deacetylase 1, also designated HD1) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.

In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and HDAC10), class III (SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6 and SIRT-7) and class IV (HDAC11).

HDAC1 and HDAC2 are recruited to Mad-Max complexes, which associate with the mSin3 scaffold protein, and are required for the transcriptional repression of Mad-Max target genes. HDAC1 is also involved in the regulation of p53. HDAC1 is expressed in various tissues. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both Histone H3 and Histone H4 in free histones or nucleosome substrate.

Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for HDAC1 antibody.

qPCR Primers

Active Motif's Human Positive Control Primer Set ACTB-2 (Cat. No. 71005) and Human Negative Control Primer Set 1 (Cat. No. 71001) have been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Positive Control Primer Set Actb-2 (Cat. No. 71017) and Mouse Negative Control Primer Set 1 (Cat. No. 71011) have been validated for both qPCR and endpoint PCR.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with HDAC1 antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting