Prox1 antibody (mAb)

Clone: 5G10
Catalog No: 61093 Format: 100 µg $435 Buy
Catalog No: 61094 Format: 10 µg $105 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
90 kDa
Reactivity:
Human

Applications

Immunofluorescence Validated Immunohistochemistry Validated Immunocytochemistry Validated

Application Notes


Published Applications:
ICC/IF
IHC
See references for more information. Individual optimization may be required.

A Western blot validated antibody is available for this target: 61091.

Immunogen

This Prox1 antibody was raised against a recombinant protein corresponding to amino acids 547-737 of human Prox1.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

Background

Prox1 (Prospero-like homeobox protein 1) is a sequence-specific DNA binding transcription factor. Prox1 controls lymphatic endothelial and retinal progenitor cell differentiation, as well as development of the liver and lens of the eye. Required for lymphatic endothelial cells specification and the formation of lymphatic vessels.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Prox1 antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting