TIEG1 / KLF10 antibody (pAb)

Aliases: EGRA, TIEG, EGR-alpha
Catalog No: 61117 Format: 100 µl $435 Buy
Catalog No: 61118 Format: 10 µl $105 Buy

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Antibody Type:
Affinity Purified
Molecular Weight:
50 kDa


Western Blot Validated

Application Notes

Validated Applications:
WB: 1:500 - 1:1,000 dilution

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.


This TIEG1 / KLF10 antibody was raised against a peptide derived from human TIEG1 / KLF10.


Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.


TIEG1 / KLF10 antibody (pAb) tested by Western blot.

Western Blot:
K-562 nuclear extract (20 µg per lane) probed with the TIEG1 / KLF10 antibody (pAb) at a dilution of 1:500.


TIEG1 / KLF10 (Transforming growth factor β-inducible early gene 1, Kruppel-like factor 10) is a transcriptional regulator involved in the control of cell growth. KLF10 binds to Sp1 / GC rich DNA sequences and can activate or repress the transcription of a number of genes. KLF10 is especially important for osteoblast function, including bone mineralization and support of osteoclast differentiation. KLF10 is rapidly induced by TGFβ, bone morphogenetic protein, estrogen and epidermal growth factor. KLF10 plays a major role in TGFβ-mediated inhibition of cell proliferation and inflammation and induction of apoptosis.

Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for KLF10 / TIEG1 antibody.


Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.


This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting