PCL2 antibody (mAb)

Clone: 3A9
Aliases: Mtf2
Catalog No: 61153 Format: 100 µg $435 Buy
Catalog No: 61154 Format: 10 µg $105 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
70 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Published Applications:
WB
See references for more information. Individual optimization may be required.

Immunogen

This PCL2 antibody was raised against a recombinant protein corresponding to amino acids 103 - 327 of mouse PCL2.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

Background

PCL2 (Polycomb-like 2, MTF2, M96) is a member of the Polycomb PRC2 protein complex and is important in cell fate decisions and may play a role in recruitment of PRC2 to target genes and histone methylation. PCL2 regulates gene expression patterns controlling self-renewal and commitment in embryonic stem cells and plays a critical role in ESC differentiation and self-renewal. PCL2 contains two PHD fingers.

Learn more about the PRC2 Complex and Stem Cell Identity

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting