BMI-1 antibody (mAb)
RRID: AB_2615070 |
Clone: AF27 |
Aliases: PCGF4, RNF51, FLVI2/BMI1 |
Catalog No: 39993 | Format: 100 µg | $530 | Add to Cart |
Catalog No: 39994 | Format: 10 µg | $130 | Add to Cart |
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Applications





Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
IP, ChIP-Seq, ChIP-qPCR
CUT&RUN
IHC(P)
ICC/IF
WB
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This BMI-1 antibody was raised against a recombinant protein corresponding to full-length mouse BMI-1.
Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
BMI-1 antibody (mAb) tested by ChIP-Seq.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from mouse embryonic fibroblast (MEF) cells and 4 µg BMI-1 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 10 million sequence tags were mapped to identify BMI-1 binding sites.
BMI-1 antibody (mAb) tested by Western blot.
20 µg of K562 cell nuclear extract was run on SDS-PAGE and probed with BMI-1 antibody at 0.5 µg/ml. For optimal results, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with BMI-1 antibody (mAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting