Phc1 antibody (mAb)

RRID: AB_2713961
Clone: 6-1-3
Aliases: EDR1, HPH1, RAE28, MCPH11
Catalog No: 39723 Format: 100 µg $445 Buy
Catalog No: 39724 Format: 10 µg $110 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
150 kDa
Reactivity:
Human, Mouse

Applications

Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes


Published Applications:
IP
ICC/IF
WB
See references for more information. Individual optimization may be required.

Immunogen

This Phc1 antibody was raised against a recombinant mouse Phc1 protein fragment.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

Background

Phc1, polyhomeotic homolog 1 (also known as EDR1, early development regulatory protein 1) is a component of the polycomb group (PcG) multiprotein PRC1 complex. The PRC1 complex is required to maintain the stable repression of the Hox genes during development. This chromatin-associated protein complex acts via chromatin remodeling and histone modifications. Phc1 has been shown to interact with PHC2, BMI1, MAPKAPK2 and MCRS1.

Learn more about the PRC1 Complex and Stem Cell Identity

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting