HIF-1 alpha antibody (mAb)

Clone: ESEE122
Aliases: HIF1A, HIF1, MOP1, PASD8, HIF-1A, bHLHe78, HIF-1alpha, HIF1-ALPHA
Catalog No: 61275 Format: 100 µg $435 Buy
Catalog No: 61276 Format: 10 µg $105 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Protein A Chromatography
Host:
Mouse
Molecular Weight:
100 kDa
Reactivity:
Bovine, Human, Mouse, Rat

Applications

Western Blot Validated Immunohistochemistry Validated

Application Notes


Published Applications:
WB
IHC (FFPE)

See references for more information. Individual optimization may be required.

Also available as AbFlex™ engineered recombinant antibodies.  For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91139.

Immunogen

This HIF-1 alpha antibody was raised against a partial protein containing amino acid residues 329-530 of human HIF-1 alpha.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

Background

HIF-1 (hypoxia inducible factor 1, alpha subunit, MOP1, PASD8, bHLHe78, PAS domain-containing protein 8) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified.

Positive Control

Active Motif's COS-7 (CoCl2 treated) nuclear extract (Catalog No. 40600) can be used as a positive control for HIF-1 alpha.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting