Histone H1.0 antibody (pAb)

RRID: AB_2793627
Catalog No: 61417 Format: 100 µl $445 Buy
Catalog No: 61418 Format: 10 µl $110 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
30 kDa
Reactivity:
Human, Mouse

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Immunogen

This antibody was raised against a peptide within the N-terminal region of human Histone H1.0.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

References

 

Histone H1.0 (pAb) tested by Western blot.
Nuclear Extract of 3T3-L1 cells (20 µg) following stimulation with a defined hormonal mixture (DMSO, dexamethasone, insulin, and IBMX) were probed with Histone H1.0 (pAb) at a dilution of 1:500.
Lane 1: Recombinant human Histone H1.0 (100 ng).
Lane 2: Day 0 - preadipocytes.
Lane 3: Day 12 - fully differentiated adipocytes.

Background

Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Histone H1.0 is a unique variant considered a replacement linker histone which is expressed and incorporated into chromatin in the absence of DNA replication. Histone H1.0 is more highly expressed in cells that are growth inhibited or terminally differentiated.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting