Mi-2 antibody (mAb)

RRID: AB_2793648
Clone: 4D8
Catalog No: 61463 Format: 100 µg $500 Buy
Catalog No: 61464 Format: 10 µg $125 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG2c
Purification:
Protein G Chromatography
Host:
Rat
Molecular Weight:
220 kDa
Reactivity:
Drosophila

Applications

Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated

Application Notes

For Mi-2 beta (CHD4), we also offer AbFlex® Mi-2 beta (CHD4) Recombinant Antibody (rAb). For details, see Catalog No. 91275.

Published Applications

The following applications have been published for clone 4D8*. Active Motif may not have validated the antibody for use in these applications:

IP
WB
IF


*Murawska et al. Mol. Cell Biol. 2008 Apr; 28(8): 2745-2757. PMID: 18250149

Immunogen

This antibody was raised against a synthetic peptide corresponding to amino acid residues 1641-1662 of Drosophila Mi-2.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

Background

Mi-2 (ATP-dependent helicase Mi-2) is a member of the CHD family of ATP-dependent chromatin remodelers characterized by the presence of two tandemly arranged chromodomains. The Chd3/Chd4 subfamily contains a pair of PHD fingers preceding the chromodomains and these proteins exist in multisubunit complexes with nucleosome remodeling and deacetylation (NuRD) activity. Drosophila Mi-2 (dMI-2) is the orthologs of mammalian CHD4.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting